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BioProcess Simulator · S. cerevisiae
KJBBIOPROCESS SIMULATION LAB

BioProcess Simulator MODEL

S. cerevisiae
ReadyEthanol production by Saccharomyces cerevisiae
20.0

15.0

10.0

5.0

0.0
O20.0 %CO20.0 % pO2100.0 %pH7.1 TR20.0 °CVolume0.0 LFoam0.0 L
Antifoam◇1.40 L0.00 ml/min
Base◇1.40 L0.00 ml/min
Acid◇1.40 L0.00 ml/min
ProductLevel  0.0 L
Thermal fluid20.0 °C
μ 0.000 h⁻¹X 0.00 g/LS 0.00 g/LP 0.00 g/LD 0.000 h⁻¹
System ready. Start a new process.Model readySamples: 0
Data:
Trends:
Measurements:

© 2026 Katharina Julia Brenner

Gas mixing station

Set each gas channel to inactive, manual or automatic operation.

Active channels follow the pO2 strategy automatically; Manual channels use their slider value.

Medium preparation

Configure initial substrate concentration and working volume.

Bioprocess model setup

Choose the operating mode, oxygen environment and kinetic parameters.

Monod kinetics
Stoichiometry
Flow strategyContinuous mode regulates the outflow around the volume setpoint. Batch disables all feed.
μ = μmax · S/(Ks + S) · fO2d(XV)/dt = μXV − FoutXd(SV)/dt = FinSin − FoutS − qSXV

Controller

Setpoints, gain and integral time directly affect the automatic loops.

Dissolved oxygen
pH
Temperature
Level
Antifoam

Process data

Current online measurements and automatically stored sample results.

Online measurements

T medium
20.0 °C
V medium
0.0 L
pH
7.1
pO2
100.0 %
O2
0.0 %
CO2
0.0 %

Last sample

Biomass total —Biomass viable —Substrate I —Substrate II —Product —

Samples are stored automatically in this browser.

No.Process timeModeX totalS IProduct
No samples recorded yet.

Online trends

Live measurements for observing and evaluating the cultivation.